2c4n

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleotide monophosphatase NagD Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AF24 (Residues: 1-250; Coverage: 100%)
Gene names: JW0661, b0675, nagD
Sequence domains:
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 53.914Å b: 94.801Å c: 119.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.218
Expression system: Escherichia coli BL21