› 2c27

X-ray diffraction
1.8Å resolution

The Structure of Mycothiol Synthase in Complex with des- AcetylMycothiol and CoenzymeA.

Released:
DOI: 10.2210/pdb2c27/pdb
PDB entry 2c27 coloured by chain, front view.
PDB entry 2c27 coloured by chain, side view.
PDB entry 2c27 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The substrate-induced conformational change of Mycobacterium tuberculosis mycothiol synthase.
Vetting MW, Yu M, Rendle PM, Blanchard JS
J Biol Chem 281 2795-802 (2006)
PMID: 16326705

Function and Biology Details

Reaction catalysed: 
Desacetylmycothiol + acetyl-CoA = CoA + mycothiol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycothiol acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 33.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJM7 (Residues: 1-315; Coverage: 100%)
Gene names: Rv0819, mshD
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

3 bound ligands:
Ligand MA8 1 x MA8
Ligand ACO 1 x ACO
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200B
Spacegroup: P21
Unit cell:
a: 37.83Å b: 59.62Å c: 61.67Å
α: 90° β: 91.57° γ: 90°
R-values:
R R work R free
0.161 0.161 0.203
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biosynthesis and functions of mycothiol, the unique protective thiol of Actinobacteria.
Newton et al. (2008)
5 more

5 mentions without citation

Bacterial GCN5-Related N-Acetyltransferases: From Resistance to Regulation.
Favrot et al. (2016)
4 more