› 2c1o

X-ray diffraction
2.75Å resolution

ENAIIHis Fab fragment in the free form

Released:
DOI: 10.2210/pdb2c1o/pdb
PDB entry 2c1o coloured by chain, front view.
PDB entry 2c1o coloured by chain, side view.
PDB entry 2c1o coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structures of an enantioselective fab-fragment in free and complex forms.
Parkkinen T, Nevanen TK, Koivula A, Rouvinen J
J Mol Biol 357 471-80 (2006)
PMID: 16427081

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212289 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, L
Molecule details ›
Chains: A, L
Length: 254 amino acids
Theoretical weight: 28.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58EU8 (Residues: 21-237; Coverage: 99%)
Gene name: Igk
Sequence domains:
Structure domains: Immunoglobulins
IGH-4 PROTEIN Chains: B, H
Molecule details ›
Chains: B, H
Length: 218 amino acids
Theoretical weight: 23.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 71.805Å b: 88.68Å c: 141.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.301
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nitrile-containing pharmaceuticals: efficacious roles of the nitrile pharmacophore.
Fleming et al. (2010)
1 more

1 mention without citation

Antibody repertoire deep sequencing reveals antigen-independent selection in maturing B cells.
Kaplinsky et al. (2014)