› 2bvg

X-ray diffraction
3.18Å resolution

Crystal structure of 6-hydoxy-D-nicotine oxidase from Arthrobacter nicotinovorans. Crystal Form 1 (P21)

Released:
DOI: 10.2210/pdb2bvg/pdb
PDB entry 2bvg coloured by chain, front view.
PDB entry 2bvg coloured by chain, side view.
PDB entry 2bvg coloured by chain, top view.
Source organism: Paenarthrobacter nicotinovorans
Primary publication:
Crystal structure of 6-hydroxy-D-nicotine oxidase from Arthrobacter nicotinovorans.
Koetter JW, Schulz GE
J Mol Biol 352 418-28 (2005)
PMID: 16095622

Function and Biology Details

Reaction catalysed: 
(1a) (R)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-6-hydroxynicotine oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 459 amino acids
Theoretical weight: 48.92 KDa
Source organism: Paenarthrobacter nicotinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08159 (Residues: 1-458; Coverage: 100%)
Gene name: 6-hdno
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 103.64Å b: 95.23Å c: 122.98Å
α: 90° β: 94.06° γ: 90°
R-values:
R R work R free
0.253 0.253 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Flavoenzymes.
Joosten et al. (2007)
7 more

2 mentions without citation

Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement.
Teufel et al. (2013)
1 more