2bvc

X-ray diffraction
2.1Å resolution

Crystal structure of Mycobacterium tuberculosis glutamine synthetase in complex with a transition state mimic

Released:
Entry authors: Krajewski WW, Jones TA, Mowbray SL

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 486 amino acids
Theoretical weight: 54.58 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WN39 (Residues: 2-478; Coverage: 100%)
Gene names: MTCY190.31, MTCY427.01, Rv2220, glnA, glnA1
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 133.261Å b: 229.457Å c: 203.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.23
Expression system: Escherichia coli BL21