› 2bsq

X-ray diffraction
3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115128 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Toxin FitB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16.33 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F882 (Residues: 1-139; Coverage: 100%)
Gene names: NGO0907, fitB, vapC
Sequence domains: PIN domain
Structure domains: 5'-nuclease
Antitoxin FitA Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 77 amino acids
Theoretical weight: 8.31 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F881 (Residues: 2-78; Coverage: 99%)
Gene names: NGO0908, fitA, vapB
Structure domains: Met repressor-like
IR36, FORWARD STRAND Chain: I
Molecule details ›
Chain: I
Length: 36 nucleotides
Theoretical weight: 11.17 KDa
IR36, REVERSE STRAND Chain: J
Molecule details ›
Chain: J
Length: 36 nucleotides
Theoretical weight: 11.2 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 2 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 75.04Å b: 82.403Å c: 135.503Å
α: 90° β: 94.19° γ: 90°
R-values:
R R work R free
0.213 0.213 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

19 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
18 more

6 mentions without citation

Structural and functional studies of the Mycobacterium tuberculosis VapBC30 toxin-antitoxin system: implications for the design of novel antimicrobial peptides.
Lee et al. (2015)
5 more