› 2bs1

X-ray diffraction
2.8Å resolution

MS2 (N87AE89K mutant) - Qbeta RNA hairpin complex

Released:
DOI: 10.2210/pdb2bs1/pdb
PDB entry 2bs1 coloured by chain, front view.
PDB entry 2bs1 coloured by chain, side view.
PDB entry 2bs1 coloured by chain, top view.
Source organism: Escherichia phage MS2
Primary publication:
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.
Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG
Structure 14 487-95 (2006)
PMID: 16531233

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137150 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 129 amino acids
Theoretical weight: 13.7 KDa
Source organism: Escherichia phage MS2
Expression system: Escherichia coli
UniProt:
  • Canonical: P03612 (Residues: 2-130; Coverage: 99%)
Sequence domains: Levivirus coat protein
Structure domains: MS2 Viral Coat Protein
5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 20 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Escherichia phage MS2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 273.1Å b: 273.5Å c: 273.4Å
α: 63.26° β: 63.11° γ: 63.31°
R-values:
R R work R free
0.245 0.245 0.248
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nucleic acid packaging in viruses.
Speir et al. (2012)
1 more

3 mentions without citation

Genetic diversity revealed by single nucleotide polymorphism markers in a worldwide germplasm collection of durum wheat.
Ren et al. (2013)
2 more