X-ray diffraction
3.3Å resolution

Crystal structure of Streptococcus Pneumoniae Hyaluronate Lyase from 70percent saturated malonate.


Function and Biology Details

Reaction catalysed:
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Hyaluronate lyase Chain: X

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 117.722Å b: 101.052Å c: 85.211Å
α: 90° β: 125.12° γ: 90°
R R work R free
0.302 0.301 0.327
Expression system: Escherichia coli