› 2bra

X-ray diffraction
2Å resolution

Structure of N-Terminal FAD Binding motif of mouse MICAL

Released:
DOI: 10.2210/pdb2bra/pdb
PDB entry 2bra coloured by chain, front view.
PDB entry 2bra coloured by chain, side view.
PDB entry 2bra coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and activity of the axon guidance protein MICAL.
Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM
Proc Natl Acad Sci U S A 102 16830-5 (2005)
PMID: 16275926

Function and Biology Details

Reactions catalysed: 
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[F-actin]-monooxygenase MICAL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 53.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VDP3 (Residues: 1-484; Coverage: 46%)
Gene names: Mical, Mical1, Nical
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 72.969Å b: 87.338Å c: 80.81Å
α: 90° β: 111.68° γ: 90°
R-values:
R R work R free
0.195 0.192 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
18 more

6 mentions without citation

MICAL, the flavoenzyme participating in cytoskeleton dynamics.
Vanoni et al. (2013)
5 more