› 2br8

X-ray diffraction
2.4Å resolution

Crystal Structure of Acetylcholine-binding Protein (AChBP) from Aplysia californica in complex with an alpha-conotoxin PnIA variant

Released:
DOI: 10.2210/pdb2br8/pdb
PDB entry 2br8 coloured by chain, front view.
PDB entry 2br8 coloured by chain, side view.
PDB entry 2br8 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an alpha-conotoxin PnIA variant.
Celie PH, Kasheverov IE, Mordvintsev DY, Hogg RC, van Nierop P, van Elk R, van Rossum-Fikkert SE, Zhmak MN, Bertrand D, Tsetlin V, Sixma TK, Smit AB
Nat Struct Mol Biol 12 582-8 (2005)
PMID: 15951818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Aplysia californica
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WSF8 (Residues: 20-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
Alpha-conotoxin PnIA Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 17 amino acids
Theoretical weight: 1.68 KDa
Source organism: Conus pennaceus
Expression system: Not provided
UniProt:
  • Canonical: P50984 (Residues: 1-16; Coverage: 100%)

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 67.473Å b: 80.085Å c: 134.043Å
α: 90° β: 93.2° γ: 90°
R-values:
R R work R free
0.201 0.198 0.25
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

41 review citations

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque et al. (2009)
40 more

40 mentions without citation

Discovery, synthesis, and structure-activity relationships of conotoxins.
Akondi et al. (2014)
39 more