› 2bol

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE AND ASSEMBLY OF TSP36, A METAZOAN SMALL HEAT SHOCK PROTEIN

Released:
DOI: 10.2210/pdb2bol/pdb
PDB entry 2bol coloured by chain, front view.
PDB entry 2bol coloured by chain, side view.
PDB entry 2bol coloured by chain, top view.
Source organism: Taenia saginata
Primary publication:
Wrapping the alpha-crystallin domain fold in a chaperone assembly.
Stamler R, Kappé G, Boelens W, Slingsby C
J Mol Biol 353 68-79 (2005)
PMID: 16165157

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small heat shock protein p36 Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.62 KDa
Source organism: Taenia saginata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7YZT0 (Residues: 1-314; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 51.59Å b: 102.05Å c: 139.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Crystallins in the eye: Function and pathology.
Andley UP. (2007)
11 more

13 mentions without citation

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
12 more