2bj8

X-ray diffraction
2.1Å resolution

NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH and LOW-AFFINITY SITES

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.83 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58316 (Residues: 1-138; Coverage: 100%)
Gene name: PH0601
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P3221
Unit cell:
a: 110.208Å b: 110.208Å c: 78.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.229
Expression system: Escherichia coli BL21(DE3)