› 2bf2

X-ray diffraction
2.1Å resolution

Crystal structure of native toluene-4-monooxygenase catalytic effector protein, T4moD

Released:
DOI: 10.2210/pdb2bf2/pdb
PDB entry 2bf2 coloured by chain, front view.
PDB entry 2bf2 coloured by chain, side view.
PDB entry 2bf2 coloured by chain, top view.
Source organism: Pseudomonas mendocina
Primary publication:
Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein.
Lountos GT, Mitchell KH, Studts JM, Fox BG, Orville AM
Biochemistry 44 7131-42 (2005)
PMID: 15882052

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toluene-4-monooxygenase system, effector component Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.5 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00459 (Residues: 2-103; Coverage: 99%)
Gene name: tmoD
Sequence domains: MmoB/DmpM family
Structure domains: Monooxygenase component MmoB/DmpM

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 86.671Å b: 86.671Å c: 144.325Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.244 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Gates of enzymes.
Gora et al. (2013)
2 more

1 mention without citation

Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase.
Liu et al. (2007)