› 2beo

X-ray diffraction
2.7Å resolution

PrfA, Transcriptional Regulator In Listeria Monocytogenes

Released:
DOI: 10.2210/pdb2beo/pdb
PDB 2beo coloured by chain and viewed from the front.
PDB 2beo coloured by chain and viewed from the side.
PDB 2beo coloured by chain and viewed from the top.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif.
Eiting M, Hagel��ken G, Schubert WD, Heinz DW
Mol. Microbiol. 56 433-46 (2005)
PMID: 15813735

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 27.2 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 2-237; Coverage: 100%)
Gene names: lmo0200, prfA
Sequence domains: Crp-like helix-turn-helix domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLN 1 x GLN
Ligand CL 2 x CL
Ligand PG4 1 x PG4
Ligand ACM 1 x ACM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 55.951Å b: 80.713Å c: 60.965Å
α: 90° β: 111.91° γ: 90°
R-values:
R R work R free
0.246 0.244 0.289
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Switching Lifestyles Is an in vivo Adaptive Strategy of Bacterial Pathogens.
Desai et al. (2019)
10 more

6 mentions without citation

The metabolic regulator CodY links Listeria monocytogenes metabolism to virulence by directly activating the virulence regulatory gene prfA.
Lobel et al. (2015)
5 more