› 2be3

X-ray diffraction
2.4Å resolution

Structure of a GTP Pyrophosphokinase Family Protein from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb2be3/pdb
PDB entry 2be3 coloured by chain, front view.
PDB entry 2be3 coloured by chain, side view.
PDB entry 2be3 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Cuff ME, Hatzos C, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RelA/SpoT domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 26.78 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UPY1 (Residues: 1-223; Coverage: 100%)
Gene name: SP_1097
Sequence domains: Region found in RelA / SpoT proteins
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand PG4 1 x PG4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 104.843Å b: 104.843Å c: 170.562Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.234
Expression system: Escherichia coli BL21(DE3)

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