› 2bd7

X-ray diffraction
1.6Å resolution

Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak)

Released:
DOI: 10.2210/pdb2bd7/pdb
PDB entry 2bd7 coloured by chain, front view.
PDB entry 2bd7 coloured by chain, side view.
PDB entry 2bd7 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural analyses on intermediates in serine protease catalysis.
Liu B, Schofield CJ, Wilmouth RC
J Biol Chem 281 24024-35 (2006)
PMID: 16754679

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133470 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
beta-casomorphin-7 Chain: P
Molecule details ›
Chain: P
Length: 7 amino acids
Theoretical weight: 864 Da
Source organism: Sus scrofa
Expression system: Not provided
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand ARG 1 x ARG
Ligand PHE 1 x PHE
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 50.043Å b: 57.705Å c: 74.357Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.211
Expression system: Not provided

Quick links

Citations

19 citation in other articles

SUMO protease SENP1 induces isomerization of the scissile peptide bond.
Shen et al. (2006)
18 more