PDBe 2bcu

X-ray diffraction
2.2Å resolution

DNA polymerase lambda in complex with a DNA duplex containing an unpaired Damp and a T:T mismatch

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase lambda Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
5'-D(P*GP*CP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*AP*GP*TP*TP*CP*G)-3' Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*GP*GP*CP*CP*GP*AP*TP*AP*CP*TP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 55.998Å b: 62.667Å c: 140.494Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.247
Expression systems:
  • Escherichia coli
  • Not provided