2bb3

X-ray diffraction
2.27Å resolution

Crystal Structure of Cobalamin Biosynthesis Precorrin-6Y Methylase (cbiE) from Archaeoglobus fulgidus

Released:
Entry authors: Kim Y, Joachimiak A, Xu X, Savchenko A, Edwards A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TP_methylase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.25 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29536 (Residues: 1-197; Coverage: 100%)
Gene name: AF_0722
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 88.191Å b: 88.418Å c: 115.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.211 0.274
Expression system: Escherichia coli BL21(DE3)