› 2bb3

X-ray diffraction
2.27Å resolution

Crystal Structure of Cobalamin Biosynthesis Precorrin-6Y Methylase (cbiE) from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2bb3/pdb
PDB 2bb3 coloured by chain and viewed from the front.
PDB 2bb3 coloured by chain and viewed from the side.
PDB 2bb3 coloured by chain and viewed from the top.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry authors: Kim Y, Joachimiak A, Xu X, Savchenko A, Edwards A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TP_methylase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.25 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29536 (Residues: 1-197; Coverage: 100%)
Gene name: AF_0722
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 88.191Å b: 88.418Å c: 115.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.211 0.274
Expression system: Escherichia coli BL21(DE3)

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