› 2b81

X-ray diffraction
2.5Å resolution

Crystal Structure of the Luciferase-like Monooxygenase from Bacillus cereus

Released:
DOI: 10.2210/pdb2b81/pdb
PDB 2b81 coloured by chain and viewed from the front.
PDB 2b81 coloured by chain and viewed from the side.
PDB 2b81 coloured by chain and viewed from the top.
Source organism: Bacillus cereus
Entry authors: Kim Y, Li H, Moy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bac_luciferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 37.44 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81B18 (Residues: 1-320; Coverage: 100%)
Gene name: BC_3378
Structure domains: Luciferase-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 39 x SO4
Ligand MES 5 x MES
Ligand GOL 5 x GOL
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 138.466Å b: 159.942Å c: 172.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.218
Expression system: Escherichia coli BL21(DE3)

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