2b3p

X-ray diffraction
1.4Å resolution

Crystal structure of a superfolder green fluorescent protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.77 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3121
Unit cell:
a: 88.456Å b: 88.456Å c: 69.346Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.218
Expression system: Escherichia coli BL21(DE3)