› 2b1c

X-ray diffraction
2.2Å resolution

5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*CP*C)-3' Zif268 binding site

Released:
DOI: 10.2210/pdb2b1c/pdb
PDB 2b1c coloured by chain and viewed from the front.
PDB 2b1c coloured by chain and viewed from the side.
PDB 2b1c coloured by chain and viewed from the top.
Source organism: Synthetic construct
Primary publication:
Signatures of protein-DNA recognition in free DNA binding sites.
Locasale JW, Napoli AA, Chen S, Berman HM, Lawson CL
J Mol Biol 386 1054-65 (2009)
PMID: 19244617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*GP*GP*TP*CP*CP*CP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.01 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6122
Unit cell:
a: 38.482Å b: 38.482Å c: 78.225Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.286
Expression system: Not provided

Quick links

Citations

1 review citation

DNA dynamics and single-molecule biology.
Duzdevich et al. (2014)
19 other articles

2 mentions without citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
1 more