2awk

X-ray diffraction
1.15Å resolution

GFP R96M mature chromophore

Released:
Source organism: Aequorea victoria
Entry authors: Wood TI, Barondeau DP, Hitomi C, Kassmann CJ, Tainer JA, Getzoff ED

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.73 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 51.235Å b: 62.35Å c: 71.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 not available 0.188
Expression system: Escherichia coli