› 2awk

X-ray diffraction
1.15Å resolution

GFP R96M mature chromophore

Released:
DOI: 10.2210/pdb2awk/pdb
PDB 2awk coloured by chain and viewed from the front.
PDB 2awk coloured by chain and viewed from the side.
PDB 2awk coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Entry authors: Wood TI, Barondeau DP, Hitomi C, Kassmann CJ, Tainer JA, Getzoff ED

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.73 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 51.235Å b: 62.35Å c: 71.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 not available 0.188
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation.
Stepanenko et al. (2013)
6 more

3 mentions without citation

Single molecule techniques in DNA repair: a primer.
Hughes et al. (2014)
2 more