2asj

X-ray diffraction
2.35Å resolution

oxoG-modified Preinsertion Binary Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.83 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 2-352; Coverage: 100%)
Gene names: SSO2448, dbh, dpo4
Sequence domains:
Structure domains:
5'-D(*GP*GP*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*(DDG))-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 13 nucleotides
Theoretical weight: 4.09 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided
5'-D(*CP*TP*AP*AP*CP*(8OG)*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3' Chains: E, J
Molecule details ›
Chains: E, J
Length: 19 nucleotides
Theoretical weight: 5.69 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P21
Unit cell:
a: 52.219Å b: 182.958Å c: 52.209Å
α: 90° β: 107.68° γ: 90°
R-values:
R R work R free
0.249 0.246 0.318
Expression systems:
  • Escherichia coli
  • Not provided