› 2ao2

X-ray diffraction
2.07Å resolution

The 2.07 Angstrom crystal structure of Mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions

Released:
DOI: 10.2210/pdb2ao2/pdb
PDB entry 2ao2 coloured by chain, front view.
PDB entry 2ao2 coloured by chain, side view.
PDB entry 2ao2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and suggests a role in host-pathogen interactions.
Qamra R, Prakash P, Aruna B, Hasnain SE, Mande SC
Biochemistry 45 6997-7005 (2006)
PMID: 16752890

Function and Biology Details

Reaction catalysed: 
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secreted chorismate mutase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 165 amino acids
Theoretical weight: 18.38 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIB9 (Residues: 35-199; Coverage: 99%)
Gene name: Rv1885c
Sequence domains: Chorismate mutase type II
Structure domains: Chorismate mutase

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 124.452Å b: 83.84Å c: 62.671Å
α: 90° β: 93.36° γ: 90°
R-values:
R R work R free
0.18 0.178 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Iron acquisition, assimilation and regulation in mycobacteria.
Banerjee et al. (2011)
1 more

5 mentions without citation

CPSARST: an efficient circular permutation search tool applied to the detection of novel protein structural relationships.
Lo et al. (2008)
4 more