› 2akm

X-ray diffraction
1.92Å resolution

Fluoride Inhibition of Enolase: Crystal Structure of the Inhibitory Complex

Released:
DOI: 10.2210/pdb2akm/pdb
PDB 2akm coloured by chain and viewed from the front.
PDB 2akm coloured by chain and viewed from the side.
PDB 2akm coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Fluoride inhibition of enolase: crystal structure and thermodynamics.
Qin J, Chai G, Brewer JM, Lovelace LL, Lebioda L
Biochemistry 45 793-800 (2006)
PMID: 16411755

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-enolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 48.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09104 (Residues: 2-434; Coverage: 100%)
Gene name: ENO2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand PO4 2 x PO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 111.739Å b: 119.692Å c: 68.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.211 0.249
Expression system: Escherichia coli

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Citations

11 review citations

Nothing lasts forever: understanding microbial biodegradation of polyfluorinated compounds and perfluorinated alkyl substances.
Wackett LP. (2022)
10 more

2 mentions without citation

Fluoride inhibition of enolase: crystal structure and thermodynamics.
Qin et al. (2006)
1 more