2aa4

X-ray diffraction
2.2Å resolution

Crystal structure of Escherichia coli putative N-ACETYLMANNOSAMINE KINASE, New York Structural Genomics Consortium

Released:
Source organism: Escherichia coli
Entry authors: Patskovsky Y, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed:
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylmannosamine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 29.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P45425 (Residues: 1-289; Coverage: 99%)
Gene names: JW5538, b3222, nanK, yhcI
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 40.991Å b: 75.841Å c: 166.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.179 0.243
Expression system: Escherichia coli BL21