› 2a9v

X-ray diffraction
2.24Å resolution

Crystal structure of a putative gmp synthase subunit a protein (ta0944m) from thermoplasma acidophilum at 2.45 A resolution

Released:
DOI: 10.2210/pdb2a9v/pdb
PDB entry 2a9v coloured by chain, front view.
PDB entry 2a9v coloured by chain, side view.
PDB entry 2a9v coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GMP synthase [glutamine-hydrolyzing] subunit A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 24.1 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HJM3 (Residues: 1-200; Coverage: 100%)
Gene names: Ta0944, guaAA
Sequence domains: Glutamine amidotransferase class-I
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand SCN 5 x SCN
Ligand GOL 7 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 275.661Å b: 39.045Å c: 68.267Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.246
Expression system: Escherichia coli

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