› 2a7c

X-ray diffraction
1.65Å resolution

On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength

Released:
DOI: 10.2210/pdb2a7c/pdb
PDB entry 2a7c coloured by chain, front view.
PDB entry 2a7c coloured by chain, side view.
PDB entry 2a7c coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength.
Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS
Acta Crystallogr D Biol Crystallogr 61 1263-72 (2005)
PMID: 16131760

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand XE 1 x XE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 50.35Å b: 58.02Å c: 74.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.182

Quick links

Citations

2 review citations

SAD phasing: History, current impact and future opportunities.
Rose et al. (2016)
1 more