2a5y

X-ray diffraction
2.6Å resolution

Structure of a CED-4/CED-9 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator ced-9 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.72 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41958 (Residues: 48-251; Coverage: 73%)
Gene names: T07C4.8, ced-9
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Cell death protein 4 Chains: B, C
Molecule details ›
Chains: B, C
Length: 549 amino acids
Theoretical weight: 62.95 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P30429 (Residues: 1-571; Coverage: 96%)
  • Best match: P30429-2 (Residues: 1-549)
Gene names: C35D10.9, ced-4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 128.898Å b: 128.898Å c: 209.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli