2a1x

X-ray diffraction
2.5Å resolution

Human phytanoyl-coa 2-hydroxylase in complex with iron and 2-oxoglutarate

Released:

Function and Biology Details

Reaction catalysed:
Phytanoyl-CoA + 2-oxoglutarate + O(2) = 2-hydroxyphytanoyl-CoA + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytanoyl-CoA dioxygenase, peroxisomal Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 35.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14832 (Residues: 31-338; Coverage: 91%)
Gene names: PAHX, PHYH
Structure domains: q2cbj1_9rhob like domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: I222
Unit cell:
a: 67.929Å b: 86.677Å c: 97.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.274
Expression system: Escherichia coli BL21(DE3)