› 2zwu

X-ray diffraction
1.3Å resolution

Crystal Structure of Camphor Soaked Ferric Cytochrome P450cam

Released:
DOI: 10.2210/pdb2zwu/pdb
PDB entry 2zwu coloured by chain, front view.
PDB entry 2zwu coloured by chain, side view.
PDB entry 2zwu coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Substrate binding induces structural changes in cytochrome P450cam.
Sakurai K, Shimada H, Hayashi T, Tsukihara T
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 80-3 (2009)
PMID: 19193991

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.72 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand CAM 1 x CAM
Ligand TRS 1 x TRS
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P43212
Unit cell:
a: 63.608Å b: 63.608Å c: 250.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.184
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

P450cam visits an open conformation in the absence of substrate.
Lee et al. (2010)
3 more

1 mention without citation

Substrate binding induces structural changes in cytochrome P450cam.
Sakurai et al. (2009)