› 2zpp

Neutron Diffraction
2.5Å resolution

Neutron crystal structure of cubic insulin at pD9

Released:
DOI: 10.2210/pdb2zpp/pdb
PDB 2zpp coloured by chain and viewed from the front.
PDB 2zpp coloured by chain and viewed from the side.
PDB 2zpp coloured by chain and viewed from the top.
Source organism: Sus scrofa
Primary publication:
An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis.
Ishikawa T, Chatake T, Morimoto Y, Maeda M, Kurihara K, Tanaka I, Niimura N
Biochem. Biophys. Res. Commun. 376 32-5 (2008)
PMID: 18725203

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS

Ligands and Environments

1 bound ligand:
Ligand DOD 34 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: I213
Unit cell:
a: 79.04Å b: 79.04Å c: 79.04Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.222 0.221 0.26

Quick links

Citations

2 review citations

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins.
O'Dell et al. (2016)
1 more

3 mentions without citation

Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.
Liebschner et al. (2018)
2 more