› 2zoi

Neutron Diffraction
1.5Å resolution

Neutron Crystal Structure of Photoactive Yellow Protein, Wild type, at 295K

Released:
DOI: 10.2210/pdb2zoi/pdb
PDB entry 2zoi coloured by chain, front view.
PDB entry 2zoi coloured by chain, side view.
PDB entry 2zoi coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Low-barrier hydrogen bond in photoactive yellow protein.
Yamaguchi S, Kamikubo H, Kurihara K, Kuroki R, Niimura N, Shimizu N, Yamazaki Y, Kataoka M
Proc Natl Acad Sci U S A 106 440-4 (2009)
PMID: 19122140

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand HC4 1 x HC4
Ligand DOD 73 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: P63
Unit cell:
a: 67.145Å b: 67.145Å c: 41.119Å α: 90° β: 90° γ: 120°
R-values:
R R work R free 0.192 0.192 0.219
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Biochemistry and theory of proton-coupled electron transfer.
Migliore et al. (2014)
9 more

19 mentions without citation

Proton transfer reactions and hydrogen-bond networks in protein environments.
Ishikita et al. (2014)
18 more