2z0d

X-ray diffraction
1.9Å resolution

The crystal structure of human Atg4B- LC3(1-120) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine protease ATG4B Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y4P1 (Residues: 1-353; Coverage: 90%)
Gene names: APG4B, ATG4B, AUTL1, KIAA0943
Sequence domains: Peptidase family C54
Microtubule-associated proteins 1A/1B light chain 3B Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 14.58 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q62625 (Residues: 1-120; Coverage: 85%)
Gene names: Map1alc3, Map1lc3, Map1lc3b
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 47.475Å b: 91.812Å c: 102.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.227
Expression system: Escherichia coli BL21