X-ray diffraction
2.11Å resolution

Crystal structure of the I-BAR domain of IRSp53 (BAIAP2) in complex with an EHEC derived Tir peptide


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Brain-specific angiogenesis inhibitor 1-associated protein 2 Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9UQB8 (Residues: 1-250; Coverage: 45%)
Gene name: BAIAP2
Sequence domains: IRSp53/MIM homology domain
Structure domains: Arfaptin homology (AH) domain/BAR domain
Translocated intimin receptor Tir Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.29 KDa
Source organism: Escherichia coli O157:H7 str. TW14359
Expression system: Not provided
  • Canonical: C6UYL8 (Residues: 452-463; Coverage: 2%)
Gene names: ECSP_4676, espE, tir

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2221
Unit cell:
a: 88.354Å b: 187.362Å c: 36.806Å
α: 90° β: 90° γ: 90°
R R work R free
0.236 0.233 0.276
Expression systems:
  • Escherichia coli
  • Not provided