2yjf

X-ray diffraction
3.5Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only tetramer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Myocardin-related transcription factor A Chain: M
Molecule details ›
Chain: M
Length: 137 amino acids
Theoretical weight: 15.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8K4J6 (Residues: 16-142; Coverage: 13%)
  • Best match: Q8K4J6-2 (Residues: 1-107)
Gene names: Bsac, Mal, Mkl1, Mrtfa
Sequence domains: RPEL repeat
Structure domains: Cobalamin-dependent Methionine Synthase; domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6522
Unit cell:
a: 182.91Å b: 182.91Å c: 378.281Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.238 0.272
Expression system: Escherichia coli BL21