X-ray diffraction
2.62Å resolution

Crystal structure of N-terminal domain of GyrA with the antibiotic simocyclinone D8


Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA gyrase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 522 amino acids
Theoretical weight: 58.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P0AES5 (Residues: 2-523; Coverage: 60%)
Gene names: S2444, SF2311, gyrA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: I212121
Unit cell:
a: 143.91Å b: 153.09Å c: 177.7Å
α: 90° β: 90° γ: 90°
R R work R free
0.204 0.202 0.241
Expression system: Escherichia coli