2y0m

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN DOSAGE COMPENSATION FACTORS MSL1 AND MOF

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase KAT8 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H7Z6 (Residues: 174-458; Coverage: 62%)
Gene names: KAT8, MOF, MYST1, PP7073
Sequence domains:
Structure domains:
Male-specific lethal 1 homolog Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6PDM1 (Residues: 470-540; Coverage: 12%)
Gene names: Msl1, Msl1l1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I4122
Unit cell:
a: 180.94Å b: 180.94Å c: 80.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.256
Expression system: Escherichia coli BL21(DE3)