PDBe 2xsw

X-ray diffraction
1.9Å resolution

Crystal structure of human INPP5E

Released:
Source organism: Homo sapiens
Entry authors: Tresaugues L, Schutz P, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kol S, Kotenyova T, Kouznetsova E, Moche M, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, Van Der Berg S, Wahlberg E, Weigelt J, Welin M, Nordlund P

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol polyphosphate 5-phosphatase type IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 40.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NRR6 (Residues: 275-623; Coverage: 54%)
Gene name: INPP5E
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 50.528Å b: 103.579Å c: 137.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.195
Expression system: Escherichia coli BL21(DE3)