2xsd

X-ray diffraction
2.05Å resolution

Crystal Structure of the dimeric Oct-6 (Pou3f1) POU domain bound to palindromic MORE DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 3, transcription factor 1 Chain: C
Molecule details ›
Chain: C
Length: 164 amino acids
Theoretical weight: 18.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21952 (Residues: 240-402; Coverage: 36%)
Gene names: Oct6, Otf-6, Otf6, Pou3f1, Scip
Sequence domains:
Structure domains:
5'-D(*AP*TP*GP*CP*AP*TP*GP*AP*GP*GP*AP)-3' Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.42 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*CP*CP*TP*CP*AP*TP*GP*CP*AP*TP*AP)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 93.497Å b: 52.009Å c: 69.013Å
α: 90° β: 129.03° γ: 90°
R-values:
R R work R free
0.198 0.194 0.232
Expression systems:
  • Escherichia coli BL21
  • Not provided