2xpi

X-ray diffraction
2.6Å resolution

Crystal structure of APC/C hetero-tetramer Cut9-Hcn1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anaphase-promoting complex subunit cut9 Chains: A, D
Molecule details ›
Chains: A, D
Length: 597 amino acids
Theoretical weight: 67.55 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P41889 (Residues: 1-597; Coverage: 89%)
Gene names: SPAC6F12.15c, cut9, dre1
Sequence domains: Anaphase-promoting complex, cyclosome, subunit 3
Structure domains: Tetratricopeptide repeat domain
Anaphase-promoting complex subunit hcn1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 81 amino acids
Theoretical weight: 9.11 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O13916 (Residues: 1-80; Coverage: 100%)
Gene names: SPAC23C11.12, hcn1
Sequence domains: Anaphase-promoting complex APC subunit CDC26

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 54.4Å b: 151.92Å c: 91.87Å
α: 90° β: 90.84° γ: 90°
R-values:
R R work R free
0.191 0.189 0.245
Expression system: Trichoplusia ni