› 2xmj

X-ray diffraction
1.08Å resolution

Visualising the Metal-binding Versatility of Copper Trafficking Sites: Atx1 side-to-side (aerobic)

Released:
DOI: 10.2210/pdb2xmj/pdb
PDB entry 2xmj coloured by chain, front view.
PDB entry 2xmj coloured by chain, side view.
PDB entry 2xmj coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Visualizing the metal-binding versatility of copper trafficking sites .
Badarau A, Firbank SJ, McCarthy AA, Banfield MJ, Dennison C
Biochemistry 49 7798-810 (2010)
PMID: 20726513

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HMA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 64 amino acids
Theoretical weight: 6.69 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73213 (Residues: 1-64; Coverage: 100%)
Gene name: ssr2857
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 63.46Å b: 41.654Å c: 55.832Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.117 not available 0.149
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.
Chen et al. (2013)
1 more