PDBe 2xl3

X-ray diffraction
2.7Å resolution

WDR5 IN COMPLEX WITH AN RBBP5 PEPTIDE AND HISTONE H3 PEPTIDE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 36.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61965 (Residues: 1-334; Coverage: 100%)
Gene names: Big, Big3, Wdr5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Retinoblastoma-binding protein 5 Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q8BX09 (Residues: 369-381; Coverage: 2%)
Gene name: Rbbp5
Histone H3.1 Chains: D, F
Molecule details ›
Chains: D, F
Length: 9 amino acids
Theoretical weight: 1.1 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P68433 (Residues: 2-9; Coverage: 6%)
Gene names: H3.1-221, H3.1-291, H3.1-I, H3a, H3g, H3h, H3i, Hist1h3a, Hist1h3g, Hist1h3h, Hist1h3i

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 47.1Å b: 81.32Å c: 86.35Å
α: 90° β: 91.13° γ: 90°
R-values:
R R work R free
0.179 0.176 0.235
Expression systems:
  • Escherichia coli
  • Not provided