› 2xdn

X-ray diffraction
2.2Å resolution

Transcription factor TtgR H67A mutant

Released:
DOI: 10.2210/pdb2xdn/pdb
PDB entry 2xdn coloured by chain, front view.
PDB entry 2xdn coloured by chain, side view.
PDB entry 2xdn coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Domain cross-talk during effector binding to the multidrug binding TTGR regulator.
Daniels C, Daddaoua A, Lu D, Zhang X, Ramos JL
J Biol Chem 285 21372-81 (2010)
PMID: 20435893

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator TtgR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 210 amino acids
Theoretical weight: 23.82 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AIU0 (Residues: 1-210; Coverage: 100%)
Gene names: T1E_0244, ttgR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 43.111Å b: 43.174Å c: 114.803Å
α: 96.93° β: 99.26° γ: 96.03°
R-values:
R R work R free
0.222 0.218 0.288
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular regulation of antibiotic biosynthesis in streptomyces.
Liu et al. (2013)
5 more

1 mention without citation

Molecular Binding Mechanism of TtgR Repressor to Antibiotics and Antimicrobials.
Fernandez-Escamilla et al. (2015)