2wj0

X-ray diffraction
3.1Å resolution

Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate

Released:
Entry authors: Carolis C, Koehler C, Sauter C, Basquin J, Suck D, Toeroe I

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Holliday junction resolvase Hjc Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.67 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28314 (Residues: 2-136; Coverage: 99%)
Gene names: AF_1965, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1
HALF-JUNCTION Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided
HALF-JUNCTION Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6322
Unit cell:
a: 110.98Å b: 110.98Å c: 150.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.202 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided