2wbs

X-ray diffraction
1.7Å resolution

Crystal structure of the zinc finger domain of Klf4 bound to its target DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Krueppel-like factor 4 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60793 (Residues: 395-483; Coverage: 18%)
Gene names: Ezf, Gklf, Klf4, Zie
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*GP*AP*GP*GP*CP*GP*CP)-3' Chain: F
Molecule details ›
Chain: F
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*GP*CP*GP*CP*CP*TP*CP)-3' Chain: G
Molecule details ›
Chain: G
Length: 7 nucleotides
Theoretical weight: 2.07 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 41Å b: 45.97Å c: 73.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.232
Expression systems:
  • Escherichia coli
  • Not provided