PDBe 2w9s

X-ray diffraction
1.8Å resolution

Staphylococcus aureus S1:DHFR in complex with trimethoprim


Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydrofolate reductase type 1 from Tn4003 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 161 amino acids
Theoretical weight: 18.5 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P13955 (Residues: 1-161; Coverage: 100%)
Gene name: dfrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

Cofactor: Ligand NDP 3 x NDP
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P2
Unit cell:
a: 115.242Å b: 67.375Å c: 115.249Å
α: 90° β: 120° γ: 90°
R R work R free
0.204 0.202 0.237
Expression system: Escherichia coli BL21(DE3)