2w5y

X-ray diffraction
2Å resolution

Binary Complex of the Mixed Lineage Leukaemia (MLL1) SET Domain with the cofactor product S-Adenosylhomocysteine.

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MLL cleavage product C180 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03164 (Residues: 3785-3969; Coverage: 5%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Sequence domains: SET domain
Structure domains: SET domain

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 48.85Å b: 56.21Å c: 78.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.264
Expression system: Escherichia coli BL21(DE3)