2w4m

X-ray diffraction
2.6Å resolution

The Crystal Structure of human N-acetylneuraminic acid phosphatase, NANP

Released:
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Cocking R, Yue WW, Pike ACW, Roos A, Muniz JRC, von Delft F, Bountra C, Arrowsmith CH, Weigelt J, Edwards A, Oppermann U

Function and Biology Details

Reaction catalysed:
N-acylneuraminate 9-phosphate + H(2)O = N-acylneuraminate + phosphate
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acylneuraminate-9-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TBE9 (Residues: 1-248; Coverage: 100%)
Gene names: C20orf147, HDHD4, NANP
Sequence domains: Haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 122.74Å b: 122.74Å c: 74.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.212
Expression system: Escherichia coli BL21(DE3)