2vxj

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH AGAL13BGAL14GLC AT 1.9 A RESOLUTION

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
PA-I galactophilic lectin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, GLA
Carbohydrate polymer : NEW Components: GAL, GLA, GLC
Carbohydrate polymer : NEW Components: GAL, GLA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 79.184Å b: 86.521Å c: 119.078Å
α: 93.93° β: 98.16° γ: 90.08°
R-values:
R R work R free
0.188 0.185 0.245
Expression system: Escherichia coli BL21(DE3)